Apple Russet Ring and Apple Green Crinkle Diseases: Fulfillment of Koch’s Postulates by Virome Analysis, Amplification of Full-Length cDNA of Viral Genomes, in vitro Transcription of Infectious Viral RNAs, and Reproduction of Symptoms on Fruits of Apple Trees Inoculated With Viral RNAs

Apple Russet Ring and Apple Green Crinkle Diseases: Fulfillment of Koch’s Postulates by Virome Analysis, Amplification of Full-Length cDNA of Viral Genomes, in vitro Transcription of Infectious Viral RNAs, and Reproduction of Symptoms on Fruits of Apple Trees Inoculated With Viral RNAs

Apple russet ring and apple green crinkle are graft-transmitted diseases first reported more than 60 years ago, but at present, no association between a specific virus (variant) and the disease has been clearly demonstrated. In this study, we conducted the following series of experiments to identify...

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Bibliographic Details
Main Authors: Chunjiang Li, Hajime Yaegashi, Ryusuke Kishigami, Ayaka Kawakubo, Noriko Yamagishi, Tsutae Ito, Nobuyuki Yoshikawa
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-07-01
Series:Frontiers in Microbiology
Subjects:
apple russet ring disease
apple green crinkle disease
next generation sequencing
apple chlorotic leaf spot virus
apple stem pitting virus
Koch’ postulates
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.01627/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2020.01627/full

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