Synthesis of Sitagliptin Intermediate by a Multi-Enzymatic Cascade System Using Lipase and Transaminase With Benzylamine as an Amino Donor

Synthesis of Sitagliptin Intermediate by a Multi-Enzymatic Cascade System Using Lipase and Transaminase With Benzylamine as an Amino Donor

Herein, we report the development of a multi-enzyme cascade using transaminase (TA), esterase, aldehyde reductase (AHR), and formate dehydrogenase (FDH), using benzylamine as an amino donor to synthesize the industrially important compound sitagliptin intermediate. A panel of 16 TAs was screened usi...

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Bibliographic Details
Main Authors: Taresh P. Khobragade, Sharad Sarak, Amol D. Pagar, Hyunwoo Jeon, Pritam Giri, Hyungdon Yun
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-10-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
sitagliptin
β-amino acid
transaminase
esterase
benzylamine
promoter engineering
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2021.757062/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2021.757062/full

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