Measuring cis-regulatory energetics in living cells using allelic manifolds
Gene expression in all organisms is controlled by cooperative interactions between DNA-bound transcription factors (TFs), but quantitatively measuring TF-DNA and TF-TF interactions remains difficult. Here we introduce a strategy for precisely measuring the Gibbs free energy of such interactions in l...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2018-12-01
|
Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/40618 |