Measuring cis-regulatory energetics in living cells using allelic manifolds

Measuring cis-regulatory energetics in living cells using allelic manifolds

Gene expression in all organisms is controlled by cooperative interactions between DNA-bound transcription factors (TFs), but quantitatively measuring TF-DNA and TF-TF interactions remains difficult. Here we introduce a strategy for precisely measuring the Gibbs free energy of such interactions in l...

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Bibliographic Details
Main Authors: Talitha L Forcier, Andalus Ayaz, Manraj S Gill, Daniel Jones, Rob Phillips, Justin B Kinney
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-12-01
Series:eLife
Subjects:
transcription
biophysics
interaction energy
CRP
reporter assay
cooperativity
Online Access:https://elifesciences.org/articles/40618

Internet

https://elifesciences.org/articles/40618

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