Development of a new barcode-based, multiplex-PCR, next-generation-sequencing assay and data processing and analytical pipeline for multiplicity of infection detection of Plasmodium falciparum

Development of a new barcode-based, multiplex-PCR, next-generation-sequencing assay and data processing and analytical pipeline for multiplicity of infection detection of Plasmodium falciparum

Abstract Background Simultaneous infection with multiple malaria parasite strains is common in high transmission areas. Quantifying the number of strains per host, or the multiplicity of infection (MOI), provides additional parasite indices for assessing transmission levels but it is challenging to...

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Bibliographic Details
Main Authors: Rebecca M. Mitchell, Zhiyong Zhou, Mili Sheth, Sheila Sergent, Michael Frace, Vishal Nayak, Bin Hu, John Gimnig, Feiko ter Kuile, Kim Lindblade, Laurence Slutsker, Mary J. Hamel, Meghna Desai, Kephas Otieno, Simon Kariuki, Ymir Vigfusson, Ya Ping Shi
Format: Article
Language:English
Published: BMC 2021-02-01
Series:Malaria Journal
Subjects:
Plasmodium falciparum
Transmission
Multiplicity of infection
Haplotype and strain
StrainRecon
MOI estimation
Online Access:https://doi.org/10.1186/s12936-021-03624-2

Internet

https://doi.org/10.1186/s12936-021-03624-2

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