Structural insights into protein folding, stability and activity using in vivo perdeuteration of hen egg-white lysozyme

Structural insights into protein folding, stability and activity using in vivo perdeuteration of hen egg-white lysozyme

This structural and biophysical study exploited a method of perdeuterating hen egg-white lysozyme based on the expression of insoluble protein in Escherichia coli followed by in-column chemical refolding. This allowed detailed comparisons with perdeuterated lysozyme produced in the yeast Pichia past...

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Bibliographic Details
Main Authors: Joao Ramos, Valerie Laux, Michael Haertlein, Elisabetta Boeri Erba, Katherine E. McAuley, V. Trevor Forsyth, Estelle Mossou, Sine Larsen, Annette E. Langkilde
Format: Article
Language:English
Published: International Union of Crystallography 2021-05-01
Series:IUCrJ
Subjects:
perdeuterated lysozyme
hewl
isotope effect
protein refolding
biophysical characterization
x-ray crystallography
protein structure
structural biology
Online Access:http://scripts.iucr.org/cgi-bin/paper?S2052252521001299

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http://scripts.iucr.org/cgi-bin/paper?S2052252521001299

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