krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells

krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells

Abstracts Background CRISPR/Cas9 system is a powerful tool for knocking out genes in cells. However, genes essential for cell survival cannot be directly knocked out. Traditionally, generation of conditional knockout cells requires multiple steps. Results In this study, we developed an easy and effi...

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Bibliographic Details
Main Authors: Bei Wang, Zishi Wang, Daqi Wang, Baolong Zhang, Sang-Ging Ong, Mingqing Li, Wenqiang Yu, Yongming Wang
Format: Article
Language:English
Published: BMC 2019-04-01
Series:Journal of Biological Engineering
Subjects:
Episomal vector
CRISPR/Cas9
Essential gene
Knockout
Online Access:http://link.springer.com/article/10.1186/s13036-019-0150-y

Internet

http://link.springer.com/article/10.1186/s13036-019-0150-y

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