Cyclization of Single-Chain Fv Antibodies Markedly Suppressed Their Characteristic Aggregation Mediated by Inter-Chain VH-VL Interactions

Cyclization of Single-Chain Fv Antibodies Markedly Suppressed Their Characteristic Aggregation Mediated by Inter-Chain VH-VL Interactions

Single-chain Fv (scFv) antibodies are recombinant proteins in which the variable regions of the heavy chain (VH) and light chain (VL) are connected by a short flexible polypeptide linker. ScFvs have the advantages of easy genetic manipulation and low-cost production using <i>Escherichia coli&l...

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Bibliographic Details
Main Authors: Soichiro Yamauchi, Yoshihiro Kobashigawa, Natsuki Fukuda, Manaka Teramoto, Yuya Toyota, Chenjiang Liu, Yuka Ikeguchi, Takashi Sato, Yuko Sato, Hiroshi Kimura, Takeshi Masuda, Sumio Ohtsuki, Kentaro Noi, Teru Ogura, Hiroshi Morioka
Format: Article
Language:English
Published: MDPI AG 2019-07-01
Series:Molecules
Subjects:
single-chain Fv
aggregation propensity
sortase A
cyclic scFv
high-speed atomic force microscopy
dynamic light scattering
Online Access:https://www.mdpi.com/1420-3049/24/14/2620

Internet

https://www.mdpi.com/1420-3049/24/14/2620

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