The CRISPR/Cas9 Minipig—A Transgenic Minipig to Produce Specific Mutations in Designated Tissues

The CRISPR/Cas9 Minipig—A Transgenic Minipig to Produce Specific Mutations in Designated Tissues

The generation of large transgenic animals is impeded by complex cloning, long maturation and gastrulation times. An introduction of multiple gene alterations increases the complexity. We have cloned a transgenic Cas9 minipig to introduce multiple mutations by CRISPR in somatic cells. Transgenic Cas...

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Bibliographic Details
Main Authors: Martin Fogtmann Berthelsen, Maria Riedel, Huiqiang Cai, Søren H. Skaarup, Aage K. O. Alstrup, Frederik Dagnæs-Hansen, Yonglun Luo, Uffe B. Jensen, Henrik Hager, Ying Liu, Henrik Callesen, Mikkel H. Vendelbo, Jannik E. Jakobsen, Martin Kristian Thomsen
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:Cancers
Subjects:
CRISPR
cancer
animal models
porcine model
lung cancer
handmade cloning
Online Access:https://www.mdpi.com/2072-6694/13/12/3024

Internet

https://www.mdpi.com/2072-6694/13/12/3024

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