Electrofusion of single cells in picoliter droplets

Abstract We present a microfluidic chip that enables electrofusion of cells in microdroplets, with exchange of nuclear components. It is shown, to our knowledge for the first time, electrofusion of two HL60 cells, inside a microdroplet. This is the crucial intermediate step for controlled hybridoma...

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Bibliographic Details
Main Authors: Rogier M. Schoeman, Wesley T. E. van den Beld, Evelien W. M. Kemna, Floor Wolbers, Jan C. T. Eijkel, Albert van den Berg
Format: Article
Language:English
Published: Nature Publishing Group 2018-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-018-21993-8