Human Intestinal Enteroids Model MHC-II in the Gut Epithelium

The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is...

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Main Authors:	Jonathan E. Wosen, Alexandra Ilstad-Minnihan, Julia Y. Co, Wei Jiang, Dhriti Mukhopadhyay, Nielsen Q. Fernandez-Becker, Calvin J. Kuo, Manuel R. Amieva, Elizabeth D. Mellins
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2019-08-01
Series:	Frontiers in Immunology
Subjects:	MHC-II epithelial cells enteroid culture antigen presentation mucosal immunity
Online Access:	https://www.frontiersin.org/article/10.3389/fimmu.2019.01970/full

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spelling	doaj-3663dc00214340a2a9175c550d4c566d2020-11-25T02:04:07ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-08-011010.3389/fimmu.2019.01970454732Human Intestinal Enteroids Model MHC-II in the Gut EpitheliumJonathan E. Wosen0Alexandra Ilstad-Minnihan1Julia Y. Co2Wei Jiang3Dhriti Mukhopadhyay4Nielsen Q. Fernandez-Becker5Calvin J. Kuo6Manuel R. Amieva7Manuel R. Amieva8Elizabeth D. Mellins9Program in Immunology, Department of Pediatrics, Stanford University, Stanford, CA, United StatesProgram in Immunology, Department of Pediatrics, Stanford University, Stanford, CA, United StatesDivision of Infectious Diseases, Department of Pediatrics, Stanford University, Stanford, CA, United StatesProgram in Immunology, Department of Pediatrics, Stanford University, Stanford, CA, United StatesProgram in Immunology, Department of Pediatrics, Stanford University, Stanford, CA, United StatesDivision of Gastroenterology and Hepatology, Department of Medicine, Stanford University, Stanford, CA, United StatesDivision of Hematology, Department of Medicine, Stanford University, Stanford, CA, United StatesDivision of Infectious Diseases, Department of Pediatrics, Stanford University, Stanford, CA, United StatesDepartment of Microbiology and Immunology, Stanford University, Stanford, CA, United StatesProgram in Immunology, Department of Pediatrics, Stanford University, Stanford, CA, United StatesThe role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel in vitro studies into IEC MHC-II regulation and function during health and disease.https://www.frontiersin.org/article/10.3389/fimmu.2019.01970/fullMHC-IIepithelial cellsenteroid cultureantigen presentationmucosal immunity
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jonathan E. Wosen Alexandra Ilstad-Minnihan Julia Y. Co Wei Jiang Dhriti Mukhopadhyay Nielsen Q. Fernandez-Becker Calvin J. Kuo Manuel R. Amieva Manuel R. Amieva Elizabeth D. Mellins
spellingShingle	Jonathan E. Wosen Alexandra Ilstad-Minnihan Julia Y. Co Wei Jiang Dhriti Mukhopadhyay Nielsen Q. Fernandez-Becker Calvin J. Kuo Manuel R. Amieva Manuel R. Amieva Elizabeth D. Mellins Human Intestinal Enteroids Model MHC-II in the Gut Epithelium Frontiers in Immunology MHC-II epithelial cells enteroid culture antigen presentation mucosal immunity
author_facet	Jonathan E. Wosen Alexandra Ilstad-Minnihan Julia Y. Co Wei Jiang Dhriti Mukhopadhyay Nielsen Q. Fernandez-Becker Calvin J. Kuo Manuel R. Amieva Manuel R. Amieva Elizabeth D. Mellins
author_sort	Jonathan E. Wosen
title	Human Intestinal Enteroids Model MHC-II in the Gut Epithelium
title_short	Human Intestinal Enteroids Model MHC-II in the Gut Epithelium
title_full	Human Intestinal Enteroids Model MHC-II in the Gut Epithelium
title_fullStr	Human Intestinal Enteroids Model MHC-II in the Gut Epithelium
title_full_unstemmed	Human Intestinal Enteroids Model MHC-II in the Gut Epithelium
title_sort	human intestinal enteroids model mhc-ii in the gut epithelium
publisher	Frontiers Media S.A.
series	Frontiers in Immunology
issn	1664-3224
publishDate	2019-08-01
description	The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel in vitro studies into IEC MHC-II regulation and function during health and disease.
topic	MHC-II epithelial cells enteroid culture antigen presentation mucosal immunity
url	https://www.frontiersin.org/article/10.3389/fimmu.2019.01970/full
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Human Intestinal Enteroids Model MHC-II in the Gut Epithelium

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