Selection-independent generation of gene knockout mouse embryonic stem cells using zinc-finger nucleases.

Selection-independent generation of gene knockout mouse embryonic stem cells using zinc-finger nucleases.

Gene knockout in murine embryonic stem cells (ESCs) has been an invaluable tool to study gene function in vitro or to generate animal models with altered phenotypes. Gene targeting using standard techniques, however, is rather inefficient and typically does not exceed frequencies of 10(-6). In conse...

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Bibliographic Details
Main Authors: Anna Osiak, Frank Radecke, Eva Guhl, Sarah Radecke, Nadine Dannemann, Fabienne Lütge, Silke Glage, Cornelia Rudolph, Tobias Cantz, Klaus Schwarz, Regine Heilbronn, Toni Cathomen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3237556?pdf=render

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http://europepmc.org/articles/PMC3237556?pdf=render

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