Endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency.
The therapeutic potential of stem cells is limited by the non-uniformity of their phenotypic state. Thus it would be advantageous to noninvasively monitor stem cell status. Driven by this challenge, we employed multidimensional multiphoton microscopy to quantify changes in endogenous fluorescence oc...
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2012-01-01
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doaj-36ef4ca9d3c44326a4d66fe5b8c8a3242020-11-25T00:23:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4370810.1371/journal.pone.0043708Endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency.Jayne M SquirrellJimmy J FongCarlos A ArizaAmber MaelKassondra MeyerNirupama K ShevdeAvtar RoopraGary E LyonsTimothy J KampKevin W EliceiriBrenda M OgleThe therapeutic potential of stem cells is limited by the non-uniformity of their phenotypic state. Thus it would be advantageous to noninvasively monitor stem cell status. Driven by this challenge, we employed multidimensional multiphoton microscopy to quantify changes in endogenous fluorescence occurring with pluripotent stem cell differentiation. We found that global and cellular-scale fluorescence lifetime of human embryonic stem cells (hESC) and murine embryonic stem cells (mESC) consistently decreased with differentiation. Less consistent were trends in endogenous fluorescence intensity with differentiation, suggesting intensity is more readily impacted by nuances of species and scale of analysis. What emerges is a practical and accessible approach to evaluate, and ultimately enrich, living stem cell populations based on changes in metabolism that could be exploited for both research and clinical applications.http://europepmc.org/articles/PMC3430704?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jayne M Squirrell Jimmy J Fong Carlos A Ariza Amber Mael Kassondra Meyer Nirupama K Shevde Avtar Roopra Gary E Lyons Timothy J Kamp Kevin W Eliceiri Brenda M Ogle |
spellingShingle |
Jayne M Squirrell Jimmy J Fong Carlos A Ariza Amber Mael Kassondra Meyer Nirupama K Shevde Avtar Roopra Gary E Lyons Timothy J Kamp Kevin W Eliceiri Brenda M Ogle Endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency. PLoS ONE |
author_facet |
Jayne M Squirrell Jimmy J Fong Carlos A Ariza Amber Mael Kassondra Meyer Nirupama K Shevde Avtar Roopra Gary E Lyons Timothy J Kamp Kevin W Eliceiri Brenda M Ogle |
author_sort |
Jayne M Squirrell |
title |
Endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency. |
title_short |
Endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency. |
title_full |
Endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency. |
title_fullStr |
Endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency. |
title_full_unstemmed |
Endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency. |
title_sort |
endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
The therapeutic potential of stem cells is limited by the non-uniformity of their phenotypic state. Thus it would be advantageous to noninvasively monitor stem cell status. Driven by this challenge, we employed multidimensional multiphoton microscopy to quantify changes in endogenous fluorescence occurring with pluripotent stem cell differentiation. We found that global and cellular-scale fluorescence lifetime of human embryonic stem cells (hESC) and murine embryonic stem cells (mESC) consistently decreased with differentiation. Less consistent were trends in endogenous fluorescence intensity with differentiation, suggesting intensity is more readily impacted by nuances of species and scale of analysis. What emerges is a practical and accessible approach to evaluate, and ultimately enrich, living stem cell populations based on changes in metabolism that could be exploited for both research and clinical applications. |
url |
http://europepmc.org/articles/PMC3430704?pdf=render |
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