The Combination of Cigarette Smoking and Alcohol Consumption Synergistically Increases Reactive Carbonyl Species in Human Male Plasma
Cigarette smoking and alcohol consumption are major risk factors for lifestyle-related diseases. Although it has been reported that the combination of these habits worsens risks, the underlying mechanism remains elusive. Reactive carbonyl species (RCS) cause chemical modifications of biological mole...
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doaj-37000fd9c8634a6fb00073a9372235012021-08-26T13:53:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-01229043904310.3390/ijms22169043The Combination of Cigarette Smoking and Alcohol Consumption Synergistically Increases Reactive Carbonyl Species in Human Male PlasmaKanae Mure0Susumu Tomono1Minae Mure2Mano Horinaka3Michihiro Mutoh4Toshiyuki Sakai5Hideki Ishikawa6Keiji Wakabayashi7Department of Public Health, Wakayama Medical University School of Medicine, Wakayama 641-8509, JapanGraduate Division of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka 422-8526, JapanDepartment of Chemistry, The University of Kansas, Lawrence, KS 66045, USADepartment of Drug Discovery Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, JapanDepartment of Molecular-Targeting Prevention, Kyoto Prefectural University of Medicine, Kyoto 602-8566, JapanDepartment of Drug Discovery Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, JapanDepartment of Molecular-Targeting Prevention, Kyoto Prefectural University of Medicine, Kyoto 602-8566, JapanGraduate Division of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka 422-8526, JapanCigarette smoking and alcohol consumption are major risk factors for lifestyle-related diseases. Although it has been reported that the combination of these habits worsens risks, the underlying mechanism remains elusive. Reactive carbonyl species (RCS) cause chemical modifications of biological molecules, leading to alterations in cellular signaling pathways, and total RCS levels have been used as a lipid peroxidation marker linked to lifestyle-related diseases. In this study, at least 41 types of RCS were identified in the lipophilic fraction of plasma samples from 40 subjects using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). Higher levels of 10 alkanals, 5 <i>trans</i>-2-alkenals, 1 <i>cis</i>-4-alkenal, and 3 alkadienals were detected in the smoking/drinking group (<i>N</i> = 10) as compared to those with either habit (<i>N</i> = 10 each) or without both habits (<i>N</i> = 10) in the analysis of covariances adjusted for age and BMI. The levels of 3 alkanals, 1 <i>trans</i>-2-alkenal, 1 alkadienal, and 1 4-hydroxy-2-alkenal in the smoking/drinking group were significantly higher than those in the no-smoking/drinking and no-smoking/no-drinking groups. These results strongly indicate that the combination of cigarette smoking and alcohol drinking synergistically increases the level and variety of RCS in the circulating blood, and may further jeopardize cellular function.https://www.mdpi.com/1422-0067/22/16/9043reactive carbonyl speciescigarette smokingalcohol drinkinghuman plasma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kanae Mure Susumu Tomono Minae Mure Mano Horinaka Michihiro Mutoh Toshiyuki Sakai Hideki Ishikawa Keiji Wakabayashi |
spellingShingle |
Kanae Mure Susumu Tomono Minae Mure Mano Horinaka Michihiro Mutoh Toshiyuki Sakai Hideki Ishikawa Keiji Wakabayashi The Combination of Cigarette Smoking and Alcohol Consumption Synergistically Increases Reactive Carbonyl Species in Human Male Plasma International Journal of Molecular Sciences reactive carbonyl species cigarette smoking alcohol drinking human plasma |
author_facet |
Kanae Mure Susumu Tomono Minae Mure Mano Horinaka Michihiro Mutoh Toshiyuki Sakai Hideki Ishikawa Keiji Wakabayashi |
author_sort |
Kanae Mure |
title |
The Combination of Cigarette Smoking and Alcohol Consumption Synergistically Increases Reactive Carbonyl Species in Human Male Plasma |
title_short |
The Combination of Cigarette Smoking and Alcohol Consumption Synergistically Increases Reactive Carbonyl Species in Human Male Plasma |
title_full |
The Combination of Cigarette Smoking and Alcohol Consumption Synergistically Increases Reactive Carbonyl Species in Human Male Plasma |
title_fullStr |
The Combination of Cigarette Smoking and Alcohol Consumption Synergistically Increases Reactive Carbonyl Species in Human Male Plasma |
title_full_unstemmed |
The Combination of Cigarette Smoking and Alcohol Consumption Synergistically Increases Reactive Carbonyl Species in Human Male Plasma |
title_sort |
combination of cigarette smoking and alcohol consumption synergistically increases reactive carbonyl species in human male plasma |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-08-01 |
description |
Cigarette smoking and alcohol consumption are major risk factors for lifestyle-related diseases. Although it has been reported that the combination of these habits worsens risks, the underlying mechanism remains elusive. Reactive carbonyl species (RCS) cause chemical modifications of biological molecules, leading to alterations in cellular signaling pathways, and total RCS levels have been used as a lipid peroxidation marker linked to lifestyle-related diseases. In this study, at least 41 types of RCS were identified in the lipophilic fraction of plasma samples from 40 subjects using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). Higher levels of 10 alkanals, 5 <i>trans</i>-2-alkenals, 1 <i>cis</i>-4-alkenal, and 3 alkadienals were detected in the smoking/drinking group (<i>N</i> = 10) as compared to those with either habit (<i>N</i> = 10 each) or without both habits (<i>N</i> = 10) in the analysis of covariances adjusted for age and BMI. The levels of 3 alkanals, 1 <i>trans</i>-2-alkenal, 1 alkadienal, and 1 4-hydroxy-2-alkenal in the smoking/drinking group were significantly higher than those in the no-smoking/drinking and no-smoking/no-drinking groups. These results strongly indicate that the combination of cigarette smoking and alcohol drinking synergistically increases the level and variety of RCS in the circulating blood, and may further jeopardize cellular function. |
topic |
reactive carbonyl species cigarette smoking alcohol drinking human plasma |
url |
https://www.mdpi.com/1422-0067/22/16/9043 |
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