Evaluation method for asymmetric uncertainty of quantitative polymerase chain reaction measurements of deoxyribonucleic acids with low copy number

Abstract Recently, in food safety and various other fields, qualitative and quantitative gene analysis using real-time polymerase chain reaction (PCR) method has become increasingly popular. The limit of detection (LOD) and quantifiable range for these measurements depends on the range and precision...

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Bibliographic Details
Main Authors: Unoh Ki, Takeru Suzuki, Satoshi Nakazawa, Yuuki Yonekawa, Kazuki Watanabe, Michie Hashimoto, Shigeo Hatada, Hirotaka Unno
Format: Article
Language:English
Published: Nature Publishing Group 2021-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-90959-0