CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation

CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation

CRISPR-Cas9 genome editing is presumed to knock out gene function by generating a frameshift during NHEJ repair. Here, the authors investigate mRNA and protein expression in edited lines and find genome editing can generate internal ribosome entry sites or alternatively spliced variants.

Bibliographic Details
Main Authors: Rubina Tuladhar, Yunku Yeu, John Tyler Piazza, Zhen Tan, Jean Rene Clemenceau, Xiaofeng Wu, Quinn Barrett, Jeremiah Herbert, David H. Mathews, James Kim, Tae Hyun Hwang, Lawrence Lum
Format: Article
Language:English
Published: Nature Publishing Group 2019-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-12028-5

Internet

https://doi.org/10.1038/s41467-019-12028-5

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