Comparative Study of Eleven Mechanical Pretreatment Protocols for <i>Cryptosporidium parvum</i> DNA Extraction from Stool Samples

Comparative Study of Eleven Mechanical Pretreatment Protocols for <i>Cryptosporidium parvum</i> DNA Extraction from Stool Samples

Nowadays, many commercial kits allow the polymerase chain reaction (PCR) detection of <i>Cryptosporidium</i> deoxyribonucleic acid (DNA) in stool samples, the efficiency of which relies on the extraction method used. Mechanical pretreatment of the stools using grinding beads has been rep...

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Bibliographic Details
Main Authors: Laure Claudel, Nicolas Valeix, Louise Basmaciyan, Bruno Pereira, Damien Costa, Anne Vincent, Stéphane Valot, Loic Favennec, Frederic Dalle
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Microorganisms
Subjects:
<i>Cryptosporidium parvum</i>
mechanical pretreatment
DNA extraction
stool samples
real-time PCR
molecular diagnosis
Online Access:https://www.mdpi.com/2076-2607/9/2/297

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https://www.mdpi.com/2076-2607/9/2/297

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