Biosynthesis of 5α-cholestan-3β-ol in rat and guinea pig liver in vitro

The in vitro conversion of dl-mevalonate-2-C14 and cholesterol-4-C14 to 5α-cholestan-3β-ol was studied in liver homogenates of rats and guinea pigs. On the average 0.01–0.05% of the substrate radioactivity was converted to 5α-cholestan-3β-ol by the livers of both species during the 4 hr incubation p...

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Main Authors:	Sarah Shefer, Susan Milch, Erwin H. Mosbach
Format:	Article
Language:	English
Published:	Elsevier 1965-01-01
Series:	Journal of Lipid Research
Subjects:	5α-cholestan-3β-ol biosynthesis in vitro liver homogenate rat guinea pig
Online Access:	http://www.sciencedirect.com/science/article/pii/S002222752039636X

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spelling	doaj-37657ac3424a4997b2545ff9f0427cb72021-04-23T06:11:53ZengElsevierJournal of Lipid Research0022-22751965-01-01613336Biosynthesis of 5α-cholestan-3β-ol in rat and guinea pig liver in vitroSarah Shefer0Susan Milch1Erwin H. Mosbach2Department of Laboratory Diagnosis, Public Health Research Institute of the City of New York, Inc., and the Bureau of Laboratories, New York City Department of Health, New York, N. Y.Department of Laboratory Diagnosis, Public Health Research Institute of the City of New York, Inc., and the Bureau of Laboratories, New York City Department of Health, New York, N. Y.Department of Laboratory Diagnosis, Public Health Research Institute of the City of New York, Inc., and the Bureau of Laboratories, New York City Department of Health, New York, N. Y.The in vitro conversion of dl-mevalonate-2-C14 and cholesterol-4-C14 to 5α-cholestan-3β-ol was studied in liver homogenates of rats and guinea pigs. On the average 0.01–0.05% of the substrate radioactivity was converted to 5α-cholestan-3β-ol by the livers of both species during the 4 hr incubation period. The biosynthesis of 5α-cholestan-3β-ol by cell-free liver homogenates in vitro demonstrates that the presence of this saturated sterol is at least partly due to biosynthesis by mammalian tissues, and cannot be ascribed exclusively to bacterial action upon cholesterol in the intestine.http://www.sciencedirect.com/science/article/pii/S002222752039636X5α-cholestan-3β-olbiosynthesisin vitroliver homogenateratguinea pig
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Sarah Shefer Susan Milch Erwin H. Mosbach
spellingShingle	Sarah Shefer Susan Milch Erwin H. Mosbach Biosynthesis of 5α-cholestan-3β-ol in rat and guinea pig liver in vitro Journal of Lipid Research 5α-cholestan-3β-ol biosynthesis in vitro liver homogenate rat guinea pig
author_facet	Sarah Shefer Susan Milch Erwin H. Mosbach
author_sort	Sarah Shefer
title	Biosynthesis of 5α-cholestan-3β-ol in rat and guinea pig liver in vitro
title_short	Biosynthesis of 5α-cholestan-3β-ol in rat and guinea pig liver in vitro
title_full	Biosynthesis of 5α-cholestan-3β-ol in rat and guinea pig liver in vitro
title_fullStr	Biosynthesis of 5α-cholestan-3β-ol in rat and guinea pig liver in vitro
title_full_unstemmed	Biosynthesis of 5α-cholestan-3β-ol in rat and guinea pig liver in vitro
title_sort	biosynthesis of 5α-cholestan-3β-ol in rat and guinea pig liver in vitro
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1965-01-01
description	The in vitro conversion of dl-mevalonate-2-C14 and cholesterol-4-C14 to 5α-cholestan-3β-ol was studied in liver homogenates of rats and guinea pigs. On the average 0.01–0.05% of the substrate radioactivity was converted to 5α-cholestan-3β-ol by the livers of both species during the 4 hr incubation period. The biosynthesis of 5α-cholestan-3β-ol by cell-free liver homogenates in vitro demonstrates that the presence of this saturated sterol is at least partly due to biosynthesis by mammalian tissues, and cannot be ascribed exclusively to bacterial action upon cholesterol in the intestine.
topic	5α-cholestan-3β-ol biosynthesis in vitro liver homogenate rat guinea pig
url	http://www.sciencedirect.com/science/article/pii/S002222752039636X
work_keys_str_mv	AT sarahshefer biosynthesisof5acholestan3bolinratandguineapigliverinvitro AT susanmilch biosynthesisof5acholestan3bolinratandguineapigliverinvitro AT erwinhmosbach biosynthesisof5acholestan3bolinratandguineapigliverinvitro
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Biosynthesis of 5α-cholestan-3β-ol in rat and guinea pig liver in vitro

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