Optimization of RNA Extraction from Rat Pancreatic Tissue
Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination....
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Shiraz University of Medical Sciences
2014-05-01
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Series: | Iranian Journal of Medical Sciences |
Subjects: | |
Online Access: | http://ijms.sums.ac.ir/index.php/IJMS/article/view/838/225 |