Optimization of RNA Extraction from Rat Pancreatic Tissue

Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination....

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Bibliographic Details
Main Authors: Sanaz Dastgheib, Cambyz Irajie, Raheleh Assaei, Farhad Koohpeima, Pooneh Mokarram
Format: Article
Language:English
Published: Shiraz University of Medical Sciences 2014-05-01
Series:Iranian Journal of Medical Sciences
Subjects:
Online Access:http://ijms.sums.ac.ir/index.php/IJMS/article/view/838/225