Improved PCR performance using template DNA from formalin-fixed and paraffin-embedded tissues by overcoming PCR inhibition.

Improved PCR performance using template DNA from formalin-fixed and paraffin-embedded tissues by overcoming PCR inhibition.

Formalin-fixed and paraffin-embedded (FFPE) tissues represent a valuable source for biomarker studies and clinical routine diagnostics. However, they suffer from degradation of nucleic acids due to the fixation process. Since genetic and epigenetic studies usually require PCR amplification, this deg...

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Bibliographic Details
Main Authors: Dimo Dietrich, Barbara Uhl, Verena Sailer, Emily Eva Holmes, Maria Jung, Sebastian Meller, Glen Kristiansen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3796491?pdf=render

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http://europepmc.org/articles/PMC3796491?pdf=render

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