Localization Microscopy of Actin Cytoskeleton in Human Platelets

Localization Microscopy of Actin Cytoskeleton in Human Platelets

Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Mor...

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Bibliographic Details
Main Authors: Sandra Mayr, Fabian Hauser, Anja Peterbauer, Andreas Tauscher, Christoph Naderer, Markus Axmann, Birgit Plochberger, Jaroslaw Jacak
Format: Article
Language:English
Published: MDPI AG 2018-04-01
Series:International Journal of Molecular Sciences
Subjects:
actin cytoskeleton
Alexa Fluor 488
drift correction
dSTORM
localization microscopy
rhodamine
photo-switching
platelet shape change
Online Access:http://www.mdpi.com/1422-0067/19/4/1150

Internet

http://www.mdpi.com/1422-0067/19/4/1150

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