Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

Direct observation of G-quadruplexes (G4s) in live cells is challenging. Here the authors report a method to identify G4s within the nuclei of live and fixed cells using a fluorescent probe combined with fluorescence lifetime imaging microscopy.

Bibliographic Details
Main Authors: Peter A. Summers, Benjamin W. Lewis, Jorge Gonzalez-Garcia, Rosa M. Porreca, Aaron H. M. Lim, Paolo Cadinu, Nerea Martin-Pintado, David J. Mann, Joshua B. Edel, Jean Baptiste Vannier, Marina K. Kuimova, Ramon Vilar
Format: Article
Language:English
Published: Nature Publishing Group 2021-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-20414-7

Internet

https://doi.org/10.1038/s41467-020-20414-7

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