Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering
Abstract Background Medaka (Oryzias latipes) is a popular animal model used in vertebrate genetic analysis. Recently, an efficient (~ 30%) knock-in system via non-homologous end joining (NHEJ) was established in zebrafish using the CRISPR/Cas9 system. If the same technique were applicable in medaka,...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2018-02-01
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Series: | Zoological Letters |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s40851-017-0086-3 |