Effects of Chemical and Mechanical Lysis on Microbial DNA Yield, Integrity, and Downstream Amplicon Sequencing of Rumen Bacteria and Protozoa

Effects of Chemical and Mechanical Lysis on Microbial DNA Yield, Integrity, and Downstream Amplicon Sequencing of Rumen Bacteria and Protozoa

QIAamp Fast DNA Stool Mini Kit (QIAGEN, Valencia, CA, United States) and RBB + C (Yu and Morrison, 2004) methodologies are widely employed to extract microbial DNA from rumen samples and can exhibit different efficiencies of obtaining DNA yield, quality, and downstream amplicon sequence analysis. No...

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Bibliographic Details
Main Authors: Zhi Yuan Ma, Xiu Min Zhang, Rong Wang, Min Wang, Ting Liu, Zhi Liang Tan
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-11-01
Series:Frontiers in Microbiology
Subjects:
chemical lysis
mechanical lysis
DNA extraction
rumen fluid
bacteria
protozoa
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2020.581227/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2020.581227/full

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