An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila

An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila

We generated two new genetic tools to efficiently tag genes in Drosophila. The first, Double Header (DH) utilizes intronic MiMIC/CRIMIC insertions to generate artificial exons for GFP mediated protein trapping or T2A-GAL4 gene trapping in vivo based on Cre recombinase to avoid embryo injections. DH...

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Bibliographic Details
Main Authors: David Li-Kroeger, Oguz Kanca, Pei-Tseng Lee, Sierra Cowan, Michael T Lee, Manish Jaiswal, Jose Luis Salazar, Yuchun He, Zhongyuan Zuo, Hugo J Bellen
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-08-01
Series:eLife
Subjects:
Double Header
protein tagging
T2A-GAL4
gene editing
Trojan exons
swappable insertion cassette
Online Access:https://elifesciences.org/articles/38709

Internet

https://elifesciences.org/articles/38709

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