Genome re-sequencing and reannotation of the Escherichia coli ER2566 strain and transcriptome sequencing under overexpression conditions

Genome re-sequencing and reannotation of the Escherichia coli ER2566 strain and transcriptome sequencing under overexpression conditions

Abstract Background The Escherichia coli ER2566 strain (NC_CP014268.2) was developed as a BL21 (DE3) derivative strain and had been widely used in recombinant protein expression. However, like many other current RefSeq annotations, the annotation of the ER2566 strain was incomplete, with missing gen...

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Bibliographic Details
Main Authors: Lizhi Zhou, Hai Yu, Kaihang Wang, Tingting Chen, Yue Ma, Yang Huang, Jiajia Li, Liqin Liu, Yuqian Li, Zhibo Kong, Qingbing Zheng, Yingbin Wang, Ying Gu, Ningshao Xia, Shaowei Li
Format: Article
Language:English
Published: BMC 2020-06-01
Series:BMC Genomics
Subjects:
Escherichia coli ER2566
Genome reannotation
Transcriptome sequencing
Engineer bacteria
Online Access:http://link.springer.com/article/10.1186/s12864-020-06818-1

Internet

http://link.springer.com/article/10.1186/s12864-020-06818-1

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