Mannan Enhances IL-12 Production by Increasing Bacterial Uptake and Endosomal Degradation in L. acidophilus and S. aureus Stimulated Dendritic Cells
The mannose receptor (MR) is a C-type lectin involved in endocytosis and with a poorly defined ability to modulate cellular activation. We investigated the effect of mannan treatment prior to stimulation of murine bone marrow-derived dendritic cells with the Gram-positive bacteria Lactobacillus acid...
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doaj-38abd59df74645c3aad118c7cc8b6c622020-11-24T22:00:07ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-11-011010.3389/fimmu.2019.02646483482Mannan Enhances IL-12 Production by Increasing Bacterial Uptake and Endosomal Degradation in L. acidophilus and S. aureus Stimulated Dendritic CellsRonja Mathiesen0Helene M. S. Eld1Juliane Sørensen2Eva Fuglsang3Lisbeth Drozd Lund4Valentina Taverniti5Valentina Taverniti6Hanne Frøkiær7Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Food, Environmental and Nutritional Science, Università degli Studi di Milano, Milan, ItalyDepartment of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, DenmarkThe mannose receptor (MR) is a C-type lectin involved in endocytosis and with a poorly defined ability to modulate cellular activation. We investigated the effect of mannan treatment prior to stimulation of murine bone marrow-derived dendritic cells with the Gram-positive bacteria Lactobacillus acidophilus NCFM (L. acidophilus) on the induction of Interleukin (IL)-12. Mannan enhanced the IL-12 production induced by L. acidophilus in a dose dependent manner (up to 230% enhancement). Additionally, mannan-enhanced IL-12 induction was also demonstrated with another Gram-positive bacteria, Staphylococcus aureus (S. aureus), while an IL-12 reducing effect was seen on Escherichia coli stimulated cells. Furthermore, the expression of Interferon β (Ifnb) was increased in cells treated with mannan prior to stimulation with L. acidophilus. The addition of mannan but not of bacteria led to endocytosis of MR, while addition of mannan prior to L. acidophilus or S. aureus resulted in increased endocytosis of bacteria, a faster killing of endocytosed bacteria, and increased reactive oxygen species production. Expression of signaling lymphocytic activation molecule (SLAMF)1 shown previously to be involved in the facilitation of endosomal degradation was upregulated by mannan but not by L. acidophilus and S. aureus. The IL-12 enhancement by mannan but not the IL-12 induced by the bacteria was abrogated by addition of inhibitors of clathrin coated pits (chlorpromazine and monodansylcadaverine). Furthermore, the addition of acid sphingomyelinase, a facilitator of ceramide raft formation, prior to addition of L. acidophilus enhanced the IL-12 production and the endocytosis of bacteria. In summary, our results show that mannan increases the IL-12 production induced by some Gram-positive bacteria through MR-endocytosis, which increases bacterial endocytosis and endosomal killing. The differential effect of MR activation on the IL-12 production induced by Gram-positive and Gram-negative bacteria may influence the immune response toward allergens and other glycoproteins.https://www.frontiersin.org/article/10.3389/fimmu.2019.02646/fulldendritic cellsGram-positive bacteriamannose receptorIL-12endosomal killingphagocytosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ronja Mathiesen Helene M. S. Eld Juliane Sørensen Eva Fuglsang Lisbeth Drozd Lund Valentina Taverniti Valentina Taverniti Hanne Frøkiær |
spellingShingle |
Ronja Mathiesen Helene M. S. Eld Juliane Sørensen Eva Fuglsang Lisbeth Drozd Lund Valentina Taverniti Valentina Taverniti Hanne Frøkiær Mannan Enhances IL-12 Production by Increasing Bacterial Uptake and Endosomal Degradation in L. acidophilus and S. aureus Stimulated Dendritic Cells Frontiers in Immunology dendritic cells Gram-positive bacteria mannose receptor IL-12 endosomal killing phagocytosis |
author_facet |
Ronja Mathiesen Helene M. S. Eld Juliane Sørensen Eva Fuglsang Lisbeth Drozd Lund Valentina Taverniti Valentina Taverniti Hanne Frøkiær |
author_sort |
Ronja Mathiesen |
title |
Mannan Enhances IL-12 Production by Increasing Bacterial Uptake and Endosomal Degradation in L. acidophilus and S. aureus Stimulated Dendritic Cells |
title_short |
Mannan Enhances IL-12 Production by Increasing Bacterial Uptake and Endosomal Degradation in L. acidophilus and S. aureus Stimulated Dendritic Cells |
title_full |
Mannan Enhances IL-12 Production by Increasing Bacterial Uptake and Endosomal Degradation in L. acidophilus and S. aureus Stimulated Dendritic Cells |
title_fullStr |
Mannan Enhances IL-12 Production by Increasing Bacterial Uptake and Endosomal Degradation in L. acidophilus and S. aureus Stimulated Dendritic Cells |
title_full_unstemmed |
Mannan Enhances IL-12 Production by Increasing Bacterial Uptake and Endosomal Degradation in L. acidophilus and S. aureus Stimulated Dendritic Cells |
title_sort |
mannan enhances il-12 production by increasing bacterial uptake and endosomal degradation in l. acidophilus and s. aureus stimulated dendritic cells |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2019-11-01 |
description |
The mannose receptor (MR) is a C-type lectin involved in endocytosis and with a poorly defined ability to modulate cellular activation. We investigated the effect of mannan treatment prior to stimulation of murine bone marrow-derived dendritic cells with the Gram-positive bacteria Lactobacillus acidophilus NCFM (L. acidophilus) on the induction of Interleukin (IL)-12. Mannan enhanced the IL-12 production induced by L. acidophilus in a dose dependent manner (up to 230% enhancement). Additionally, mannan-enhanced IL-12 induction was also demonstrated with another Gram-positive bacteria, Staphylococcus aureus (S. aureus), while an IL-12 reducing effect was seen on Escherichia coli stimulated cells. Furthermore, the expression of Interferon β (Ifnb) was increased in cells treated with mannan prior to stimulation with L. acidophilus. The addition of mannan but not of bacteria led to endocytosis of MR, while addition of mannan prior to L. acidophilus or S. aureus resulted in increased endocytosis of bacteria, a faster killing of endocytosed bacteria, and increased reactive oxygen species production. Expression of signaling lymphocytic activation molecule (SLAMF)1 shown previously to be involved in the facilitation of endosomal degradation was upregulated by mannan but not by L. acidophilus and S. aureus. The IL-12 enhancement by mannan but not the IL-12 induced by the bacteria was abrogated by addition of inhibitors of clathrin coated pits (chlorpromazine and monodansylcadaverine). Furthermore, the addition of acid sphingomyelinase, a facilitator of ceramide raft formation, prior to addition of L. acidophilus enhanced the IL-12 production and the endocytosis of bacteria. In summary, our results show that mannan increases the IL-12 production induced by some Gram-positive bacteria through MR-endocytosis, which increases bacterial endocytosis and endosomal killing. The differential effect of MR activation on the IL-12 production induced by Gram-positive and Gram-negative bacteria may influence the immune response toward allergens and other glycoproteins. |
topic |
dendritic cells Gram-positive bacteria mannose receptor IL-12 endosomal killing phagocytosis |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.02646/full |
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