Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that monitors Ca2+fluxes in darkness without excitation.
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2016-10-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms13268 |