Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing

Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing

The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that monitors Ca2+fluxes in darkness without excitation.

Bibliographic Details
Main Authors: Jie Yang, Derrick Cumberbatch, Samuel Centanni, Shu-qun Shi, Danny Winder, Donna Webb, Carl Hirschie Johnson
Format: Article
Language:English
Published: Nature Publishing Group 2016-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms13268
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spelling doaj-38ae3b777e7041d28342246b480b0c172021-05-11T11:23:10ZengNature Publishing GroupNature Communications2041-17232016-10-017111010.1038/ncomms13268Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensingJie Yang0Derrick Cumberbatch1Samuel Centanni2Shu-qun Shi3Danny Winder4Donna Webb5Carl Hirschie Johnson6Department of Biological Sciences, Vanderbilt UniversityDepartment of Biological Sciences, Vanderbilt UniversityDepartment of Molecular Physiology and Biophysics, Vanderbilt UniversityDepartment of Biological Sciences, Vanderbilt UniversityDepartment of Molecular Physiology and Biophysics, Vanderbilt UniversityDepartment of Biological Sciences, Vanderbilt UniversityDepartment of Biological Sciences, Vanderbilt UniversityThe coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that monitors Ca2+fluxes in darkness without excitation.https://doi.org/10.1038/ncomms13268
collection DOAJ
language English
format Article
sources DOAJ
author Jie Yang
Derrick Cumberbatch
Samuel Centanni
Shu-qun Shi
Danny Winder
Donna Webb
Carl Hirschie Johnson
spellingShingle Jie Yang
Derrick Cumberbatch
Samuel Centanni
Shu-qun Shi
Danny Winder
Donna Webb
Carl Hirschie Johnson
Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
Nature Communications
author_facet Jie Yang
Derrick Cumberbatch
Samuel Centanni
Shu-qun Shi
Danny Winder
Donna Webb
Carl Hirschie Johnson
author_sort Jie Yang
title Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
title_short Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
title_full Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
title_fullStr Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
title_full_unstemmed Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
title_sort coupling optogenetic stimulation with nanoluc-based luminescence (bret) ca++ sensing
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2016-10-01
description The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that monitors Ca2+fluxes in darkness without excitation.
url https://doi.org/10.1038/ncomms13268
work_keys_str_mv AT jieyang couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing
AT derrickcumberbatch couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing
AT samuelcentanni couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing
AT shuqunshi couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing
AT dannywinder couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing
AT donnawebb couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing
AT carlhirschiejohnson couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing
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