The Chemical Composition of Achillea wilhelmsii C. Koch and Its Desirable Effects on Hyperglycemia, Inflammatory Mediators and Hypercholesterolemia as Risk Factors for Cardiometabolic Disease
This study was done to identify the content compounds of Achillea wilhelmsii (A. wilhelmsii) and to evaluate its hypoglycemic and anti-hypercholesterolemic activity and effect on inflammatory mediators. The extracts and fractions of A. wilhelmsii were thoroughly analyzed using high performance liqui...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2016-03-01
|
Series: | Molecules |
Subjects: | |
Online Access: | http://www.mdpi.com/1420-3049/21/4/404 |
id |
doaj-392fab04c114485ba61449c805e72e78 |
---|---|
record_format |
Article |
spelling |
doaj-392fab04c114485ba61449c805e72e782020-11-25T00:13:09ZengMDPI AGMolecules1420-30492016-03-0121440410.3390/molecules21040404molecules21040404The Chemical Composition of Achillea wilhelmsii C. Koch and Its Desirable Effects on Hyperglycemia, Inflammatory Mediators and Hypercholesterolemia as Risk Factors for Cardiometabolic DiseaseElian Khazneh0Petra Hřibová1Jan Hošek2Pavel Suchý3Peter Kollár4Gabriela Pražanová5Jan Muselík6Zuzana Hanaková7Jiří Václavík8Michał Miłek9Jaroslav Legáth10Karel Šmejkal11Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1, Brno 61242, Czech RepublicDepartment of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1, Brno 61242, Czech RepublicDepartment of Molecular Biology and Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1, Brno 61242, Czech RepublicDepartment of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1, Brno 61242, Czech RepublicDepartment of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1, Brno 61242, Czech RepublicDepartment of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1, Brno 61242, Czech RepublicDepartment of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, Brno 61242, Czech RepublicDepartment of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1, Brno 61242, Czech RepublicDepartment of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1, Brno 61242, Czech RepublicDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszów University of Technology, Powstańców Warszawy 6, Rzeszów 35-959, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszów University of Technology, Powstańców Warszawy 6, Rzeszów 35-959, PolandDepartment of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1, Brno 61242, Czech RepublicThis study was done to identify the content compounds of Achillea wilhelmsii (A. wilhelmsii) and to evaluate its hypoglycemic and anti-hypercholesterolemic activity and effect on inflammatory mediators. The extracts and fractions of A. wilhelmsii were thoroughly analyzed using high performance liquid chromatography (HPLC), and the total content of phenols and flavonoids was determined. The hypoglycemic activity was evaluated in vivo using alloxan-induced diabetic mice. The effect upon inflammatory mediators was evaluated in vitro using the human monocytic leukemia cell line (THP-1). The anti-hypercholesterolemic activity was evaluated in vitro using the 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase assay kit. The water extract (WE)-treated group showed the highest reduction in the fasting blood glucose levels (FBGL). The chloroform fraction (CF) and ethyl acetate fraction (EAF) both showed a significant ability to reduce the secretion of tumor necrosis factor alpha (TNF-α). The EAF, however, also attenuated the levels of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9). The CF showed the most significant 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) inhibition activity. The five main compounds in the CF were isolated and identified. Out of the five compounds in the CF, 1β,10β-epoxydesacetoxymatricarin (CP1) and leucodin (CP2) showed the highest anti-hypercholesterolemic potential. A molecular docking study provided corresponding results.http://www.mdpi.com/1420-3049/21/4/404Achillea wilhelmsiianti-hypercholesterolemiccardiometabolic diseasedockingflavonoids3-hydroxy-3-methylglutaryl-CoA reductasehypoglycemicinflammation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elian Khazneh Petra Hřibová Jan Hošek Pavel Suchý Peter Kollár Gabriela Pražanová Jan Muselík Zuzana Hanaková Jiří Václavík Michał Miłek Jaroslav Legáth Karel Šmejkal |
spellingShingle |
Elian Khazneh Petra Hřibová Jan Hošek Pavel Suchý Peter Kollár Gabriela Pražanová Jan Muselík Zuzana Hanaková Jiří Václavík Michał Miłek Jaroslav Legáth Karel Šmejkal The Chemical Composition of Achillea wilhelmsii C. Koch and Its Desirable Effects on Hyperglycemia, Inflammatory Mediators and Hypercholesterolemia as Risk Factors for Cardiometabolic Disease Molecules Achillea wilhelmsii anti-hypercholesterolemic cardiometabolic disease docking flavonoids 3-hydroxy-3-methylglutaryl-CoA reductase hypoglycemic inflammation |
author_facet |
Elian Khazneh Petra Hřibová Jan Hošek Pavel Suchý Peter Kollár Gabriela Pražanová Jan Muselík Zuzana Hanaková Jiří Václavík Michał Miłek Jaroslav Legáth Karel Šmejkal |
author_sort |
Elian Khazneh |
title |
The Chemical Composition of Achillea wilhelmsii C. Koch and Its Desirable Effects on Hyperglycemia, Inflammatory Mediators and Hypercholesterolemia as Risk Factors for Cardiometabolic Disease |
title_short |
The Chemical Composition of Achillea wilhelmsii C. Koch and Its Desirable Effects on Hyperglycemia, Inflammatory Mediators and Hypercholesterolemia as Risk Factors for Cardiometabolic Disease |
title_full |
The Chemical Composition of Achillea wilhelmsii C. Koch and Its Desirable Effects on Hyperglycemia, Inflammatory Mediators and Hypercholesterolemia as Risk Factors for Cardiometabolic Disease |
title_fullStr |
The Chemical Composition of Achillea wilhelmsii C. Koch and Its Desirable Effects on Hyperglycemia, Inflammatory Mediators and Hypercholesterolemia as Risk Factors for Cardiometabolic Disease |
title_full_unstemmed |
The Chemical Composition of Achillea wilhelmsii C. Koch and Its Desirable Effects on Hyperglycemia, Inflammatory Mediators and Hypercholesterolemia as Risk Factors for Cardiometabolic Disease |
title_sort |
chemical composition of achillea wilhelmsii c. koch and its desirable effects on hyperglycemia, inflammatory mediators and hypercholesterolemia as risk factors for cardiometabolic disease |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2016-03-01 |
description |
This study was done to identify the content compounds of Achillea wilhelmsii (A. wilhelmsii) and to evaluate its hypoglycemic and anti-hypercholesterolemic activity and effect on inflammatory mediators. The extracts and fractions of A. wilhelmsii were thoroughly analyzed using high performance liquid chromatography (HPLC), and the total content of phenols and flavonoids was determined. The hypoglycemic activity was evaluated in vivo using alloxan-induced diabetic mice. The effect upon inflammatory mediators was evaluated in vitro using the human monocytic leukemia cell line (THP-1). The anti-hypercholesterolemic activity was evaluated in vitro using the 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase assay kit. The water extract (WE)-treated group showed the highest reduction in the fasting blood glucose levels (FBGL). The chloroform fraction (CF) and ethyl acetate fraction (EAF) both showed a significant ability to reduce the secretion of tumor necrosis factor alpha (TNF-α). The EAF, however, also attenuated the levels of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9). The CF showed the most significant 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) inhibition activity. The five main compounds in the CF were isolated and identified. Out of the five compounds in the CF, 1β,10β-epoxydesacetoxymatricarin (CP1) and leucodin (CP2) showed the highest anti-hypercholesterolemic potential. A molecular docking study provided corresponding results. |
topic |
Achillea wilhelmsii anti-hypercholesterolemic cardiometabolic disease docking flavonoids 3-hydroxy-3-methylglutaryl-CoA reductase hypoglycemic inflammation |
url |
http://www.mdpi.com/1420-3049/21/4/404 |
work_keys_str_mv |
AT eliankhazneh thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT petrahribova thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT janhosek thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT pavelsuchy thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT peterkollar thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT gabrielaprazanova thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT janmuselik thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT zuzanahanakova thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT jirivaclavik thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT michałmiłek thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT jaroslavlegath thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT karelsmejkal thechemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT eliankhazneh chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT petrahribova chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT janhosek chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT pavelsuchy chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT peterkollar chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT gabrielaprazanova chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT janmuselik chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT zuzanahanakova chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT jirivaclavik chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT michałmiłek chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT jaroslavlegath chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease AT karelsmejkal chemicalcompositionofachilleawilhelmsiickochanditsdesirableeffectsonhyperglycemiainflammatorymediatorsandhypercholesterolemiaasriskfactorsforcardiometabolicdisease |
_version_ |
1725396122498760704 |