A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

Abstract We have developed an imaging method designated as correlative light microscopy and block-face imaging (CoMBI), which contributes to improve the reliability of morphological analyses. This method can collect both the frozen sections and serial block-face images in a single specimen. The froz...

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Bibliographic Details
Main Authors: Yuki Tajika, Tohru Murakami, Keiya Iijima, Hiroki Gotoh, Maiko Takahashi-Ikezawa, Hitoshi Ueno, Yuhei Yoshimoto, Hiroshi Yorifuji
Format: Article
Language:English
Published: Nature Publishing Group 2017-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-03900-9