Standardization and validation of real time PCR assays for the diagnosis of histoplasmosis using three molecular targets in an animal model.

Standardization and validation of real time PCR assays for the diagnosis of histoplasmosis using three molecular targets in an animal model.

Histoplasmosis is considered one of the most important endemic and systemic mycoses worldwide. Until now few molecular techniques have been developed for its diagnosis. The aim of this study was to develop and evaluate three real time PCR (qPCR) protocols for different protein-coding genes (100-kDa,...

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Main Authors: Luisa F López, César O Muñoz, Diego H Cáceres, Ángela M Tobón, Vladimir Loparev, Oliver Clay, Tom Chiller, Anastasia Litvintseva, Lalitha Gade, Ángel González, Beatriz L Gómez
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5747470?pdf=render

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http://europepmc.org/articles/PMC5747470?pdf=render

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