LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation
A rapid and effective method using QuEChERS-based sample preparation procedure and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis has been developed and validated to determine progesterone in rabbit plasma. The analyte was extracted from plasma by acetonitrile with phase partitio...
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Series: | Journal of Analytical Methods in Chemistry |
Online Access: | http://dx.doi.org/10.1155/2020/8889375 |
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doaj-39c6fac2f65a4c2f8da59bd78e4d20f22020-11-25T04:07:52ZengHindawi LimitedJournal of Analytical Methods in Chemistry2090-88732020-01-01202010.1155/2020/88893758889375LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal FormulationCao-Son Tran0Quang-Dong Bui1Ngoc-Tho Thi Nguyen2Minh-Hanh Dao3Thach-Tung Nguyen4Laboratory of Food Toxicology and Allergens TestingLaboratory of Food Toxicology and Allergens TestingDepartment of PharmaceuticsDepartment of PharmaceuticsDepartment of PharmaceuticsA rapid and effective method using QuEChERS-based sample preparation procedure and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis has been developed and validated to determine progesterone in rabbit plasma. The analyte was extracted from plasma by acetonitrile with phase partitioning by a mixture of magnesium sulfate and sodium chloride. The supernatant was then directly injected into LC-MS/MS in a positive electrospray ionization mode and quantified using progesterone-d9 as the internal standard. The method linearity was in the range from 1 ng/mL (LOQ) to 200 ng/mL. Method recovery was from 86.0% to 103%, and repeatability was lower than 5.5%. The plasma sample was stable for 12 weeks stored at 18 ± 2°C. This method was applied to quantify progesterone in rabbit plasma in a pharmacokinetic study of two transdermal formulations: a reference drug and a eutectic-hydrogel system. The data indicate that the eutectic-hydrogel system’s bioavailability was 1.5 times better than that of the reference drug, and the transdermal system is a potential drug delivery system for progesterone.http://dx.doi.org/10.1155/2020/8889375 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cao-Son Tran Quang-Dong Bui Ngoc-Tho Thi Nguyen Minh-Hanh Dao Thach-Tung Nguyen |
spellingShingle |
Cao-Son Tran Quang-Dong Bui Ngoc-Tho Thi Nguyen Minh-Hanh Dao Thach-Tung Nguyen LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation Journal of Analytical Methods in Chemistry |
author_facet |
Cao-Son Tran Quang-Dong Bui Ngoc-Tho Thi Nguyen Minh-Hanh Dao Thach-Tung Nguyen |
author_sort |
Cao-Son Tran |
title |
LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation |
title_short |
LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation |
title_full |
LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation |
title_fullStr |
LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation |
title_full_unstemmed |
LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation |
title_sort |
lc-ms/ms method for rapid quantification of progesterone in rabbit plasma and its application in a pharmacokinetic study of the transdermal formulation |
publisher |
Hindawi Limited |
series |
Journal of Analytical Methods in Chemistry |
issn |
2090-8873 |
publishDate |
2020-01-01 |
description |
A rapid and effective method using QuEChERS-based sample preparation procedure and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis has been developed and validated to determine progesterone in rabbit plasma. The analyte was extracted from plasma by acetonitrile with phase partitioning by a mixture of magnesium sulfate and sodium chloride. The supernatant was then directly injected into LC-MS/MS in a positive electrospray ionization mode and quantified using progesterone-d9 as the internal standard. The method linearity was in the range from 1 ng/mL (LOQ) to 200 ng/mL. Method recovery was from 86.0% to 103%, and repeatability was lower than 5.5%. The plasma sample was stable for 12 weeks stored at 18 ± 2°C. This method was applied to quantify progesterone in rabbit plasma in a pharmacokinetic study of two transdermal formulations: a reference drug and a eutectic-hydrogel system. The data indicate that the eutectic-hydrogel system’s bioavailability was 1.5 times better than that of the reference drug, and the transdermal system is a potential drug delivery system for progesterone. |
url |
http://dx.doi.org/10.1155/2020/8889375 |
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