Validation of a PNA Clamping Method for Reducing Host DNA Amplification and Increasing Eukaryotic Diversity in Rhizosphere Microbiome Studies
Protists and microscopic animals are important but poorly understood determinants of plant health. Plant-associated eukaryotes could be surveyed by high-throughput sequencing of 18S ribosomal RNA (rRNA) genes but the abundance of plant DNA in rhizosphere samples makes 18S rRNA gene amplification wit...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2020-10-01
|
| Series: | Phytobiomes Journal |
| Online Access: | https://doi.org/10.1094/PBIOMES-05-20-0040-TA |