CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations

CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations

CRISPR-Cas9 has been rapidly adopted to generate cell line models of disease. Here the authors show, while attempting to establish a congenital erythropoietic porphyria model, unexpected chromosome truncations generated by a p53-dependent mechanism.

Bibliographic Details
Main Authors: Grégoire Cullot, Julian Boutin, Jérôme Toutain, Florence Prat, Perrine Pennamen, Caroline Rooryck, Martin Teichmann, Emilie Rousseau, Isabelle Lamrissi-Garcia, Véronique Guyonnet-Duperat, Alice Bibeyran, Magalie Lalanne, Valérie Prouzet-Mauléon, Béatrice Turcq, Cécile Ged, Jean-Marc Blouin, Emmanuel Richard, Sandrine Dabernat, François Moreau-Gaudry, Aurélie Bedel
Format: Article
Language:English
Published: Nature Publishing Group 2019-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-09006-2

Internet

https://doi.org/10.1038/s41467-019-09006-2

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