An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells

An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells

Abstract Background Exosomes are nanovesicles (30–120 nm) of endosomal origin. These exosomes contain various functional proteins and RNAs that could be used for therapeutic purposes. Currently, having a standard method for exosome isolation retaining its biological properties with increased yield a...

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Bibliographic Details
Main Authors: Suchi Gupta, Sonali Rawat, Vivek Arora, Sarat Kumar Kottarath, Amit Kumar Dinda, Pradeep Kumar Vaishnav, Baibaswata Nayak, Sujata Mohanty
Format: Article
Language:English
Published: BMC 2018-07-01
Series:Stem Cell Research & Therapy
Subjects:
Mesenchymal stem cells
Exosomes
Sucrose cushion ultracentrifugation
Online Access:http://link.springer.com/article/10.1186/s13287-018-0923-0

Internet

http://link.springer.com/article/10.1186/s13287-018-0923-0

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