Development and evaluation of a duplex TaqMan qPCR assay for detection and quantification of Trypanosoma cruzi infection in domestic and sylvatic reservoir hosts

Development and evaluation of a duplex TaqMan qPCR assay for detection and quantification of Trypanosoma cruzi infection in domestic and sylvatic reservoir hosts

Abstract Background A question of epidemiological relevance in Chagas disease studies is to understand Trypanosoma cruzi transmission cycles and trace the origins of (re)emerging cases in areas under vector or disease surveillance. Conventional parasitological methods lack sensitivity whereas molecu...

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Bibliographic Details
Main Authors: Diana P. Wehrendt, Andrea Gómez-Bravo, Juan C. Ramirez, Carolina Cura, Angélica Pech-May, Janine M. Ramsey, Marcelo Abril, Felipe Guhl, Alejandro G. Schijman
Format: Article
Language:English
Published: BMC 2019-11-01
Series:Parasites & Vectors
Subjects:
Trypanosoma cruzi
Chagas disease
Mammalian reservoirs
Molecular epidemiology
Internal amplification standard
Multiplex qPCR
Online Access:https://doi.org/10.1186/s13071-019-3817-9

Internet

https://doi.org/10.1186/s13071-019-3817-9

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