A novel method to rescue and culture duck Astrovirus type 1 in vitro

A novel method to rescue and culture duck Astrovirus type 1 in vitro

Abstract Background Reverse genetics systems enable the manipulation of viral genomes and therefore serve as robust reverse genetic tools to study RNA viruses. A DNA-launched rescue system initiates the transcription of viral genomic cDNA from eukaryotic promoter in transfected cells, generating hom...

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Bibliographic Details
Main Authors: Ruihua Zhang, Jingjing Lan, Haie Li, Junhao Chen, Yupeng Yang, Shaoli Lin, Zhijing Xie, Shijin Jiang
Format: Article
Language:English
Published: BMC 2019-09-01
Series:Virology Journal
Subjects:
DAstV-1
DNA-launched infectious clone
Propagation
Trypsin
Online Access:http://link.springer.com/article/10.1186/s12985-019-1218-5

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http://link.springer.com/article/10.1186/s12985-019-1218-5

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