A novel method to rescue and culture duck Astrovirus type 1 in vitro
Abstract Background Reverse genetics systems enable the manipulation of viral genomes and therefore serve as robust reverse genetic tools to study RNA viruses. A DNA-launched rescue system initiates the transcription of viral genomic cDNA from eukaryotic promoter in transfected cells, generating hom...
Main Authors: | Ruihua Zhang, Jingjing Lan, Haie Li, Junhao Chen, Yupeng Yang, Shaoli Lin, Zhijing Xie, Shijin Jiang |
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Format: | Article |
Language: | English |
Published: |
BMC
2019-09-01
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Series: | Virology Journal |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12985-019-1218-5 |
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