Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil

Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil

Abstract: INTRODUCTION: The genus Flavivirus includes several pathogenic species that cause severe illness in humans. Therefore, a rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great importance. Here, we evaluate and optimize a quantitative real-tim...

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Bibliographic Details
Main Authors: Marilia Farignoli Romeiro, William Marciel de Souza, Aline Lavado Tolardo, Luiz Carlos Vieira, Tatiana Elias Colombo, Victor Hugo Aquino, Maurício Lacerda Nogueira, Luiz Tadeu Moraes Figueiredo
Format: Article
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2016-06-01
Series:Revista da Sociedade Brasileira de Medicina Tropical
Subjects:
Flavivirus
Arbovirus
Real-time RT-PCR
Diagnosis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000300279&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000300279&lng=en&tlng=en

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