Validation of Reference Genes for Normalization Gene Expression in Reverse Transcription Quantitative PCR in Human Normal Thyroid and Goiter Tissue

Reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been recognized as the most accurate method for quantifying mRNA transcripts, but normalization of samples is a prerequisite for correct data interpretation. So, this study aimed to evaluate the most stable reference gene for...

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Bibliographic Details
Main Authors: Raquel Weber, Ana Paula Santin Bertoni, Laura Walter Bessestil, Beatriz Maria de Azevedo Assis Brasil, llma Simoni Brum, Tania Weber Furlanetto
Format: Article
Language:English
Published: Hindawi Limited 2014-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2014/198582