Validation of Reference Genes for Normalization Gene Expression in Reverse Transcription Quantitative PCR in Human Normal Thyroid and Goiter Tissue
Reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been recognized as the most accurate method for quantifying mRNA transcripts, but normalization of samples is a prerequisite for correct data interpretation. So, this study aimed to evaluate the most stable reference gene for...
Main Authors: | Raquel Weber, Ana Paula Santin Bertoni, Laura Walter Bessestil, Beatriz Maria de Azevedo Assis Brasil, llma Simoni Brum, Tania Weber Furlanetto |
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2014-01-01
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Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2014/198582 |
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