Molecular cloning, expression, and functional characterization of the β-agarase AgaB-4 from Paenibacillus agarexedens

Molecular cloning, expression, and functional characterization of the β-agarase AgaB-4 from Paenibacillus agarexedens

Abstract In this study, a β-agarase gene, agaB-4, was isolated for the first time from the agar-degrading bacterium Paenibacillus agarexedens BCRC 17346 by using next-generation sequencing. agaB-4 consists of 2652 bp and encodes an 883-amino acid protein with an 18-amino acid signal peptide. agaB-4...

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Bibliographic Details
Main Authors: Zeng-Weng Chen, Hui-Jie Lin, Wen-Cheng Huang, Shih-Ling Hsuan, Jiunn-Horng Lin, Jyh-Perng Wang
Format: Article
Language:English
Published: SpringerOpen 2018-03-01
Series:AMB Express
Subjects:
β-Agarase
Paenibacillus agarexedens
Next-generation sequencing
Neoagarotetraose
Online Access:http://link.springer.com/article/10.1186/s13568-018-0581-8

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http://link.springer.com/article/10.1186/s13568-018-0581-8

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