Protocol to establish a lung adenocarcinoma immunotherapy allograft mouse model with FACS and immunofluorescence-based analysis of tumor response

Protocol to establish a lung adenocarcinoma immunotherapy allograft mouse model with FACS and immunofluorescence-based analysis of tumor response

Summary: Anti-PD-1/PD-L1 therapy shows long-term effects in many cancer types, but resistance and relapse remain the main limitations of this therapy. Here, we describe a protocol to evaluate the tumor response to immunotherapy in a mouse lung cancer model. The protocol includes the establishment of...

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Bibliographic Details
Main Authors: Zhengxin Peng, Man Yu, Jiaming Lin, Tianqi Dong, Xiao Zhang, Mingjun Shi, Min Qin, Shasha Li, Wencong Guo, Huixia Zhang, Shuguo Sun
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell biology
Cell culture
Cell isolation
Flow cytometry/mass cytometry
Cancer
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003026

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http://www.sciencedirect.com/science/article/pii/S2666166721003026

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