Effect of Bucillamine on Free-Radical-Mediated Degradation of High-Molar-Mass Hyaluronan Induced in vitro by Ascorbic Acid and Cu(II) Ions
The bucillamine effect on free-radical-mediated degradation of high-molar-mass hyaluronan (HA) has been elucidated. As HA fragmentation is expected to decrease its dynamic viscosity, rotational viscometry was applied to follow the oxidative HA degradation. Non-isothermal chemiluminometry, thermograv...
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doaj-3b9fcba27c614db88dcfc4c63d823fde2020-11-24T23:32:05ZengMDPI AGPolymers2073-43602014-10-016102625264410.3390/polym6102625polym6102625Effect of Bucillamine on Free-Radical-Mediated Degradation of High-Molar-Mass Hyaluronan Induced in vitro by Ascorbic Acid and Cu(II) IonsMária Baňasová0Katarína Valachová1Jozef Rychlý2Ivica Janigová3Katarína Csomorová4Raniero Mendichi5Danica Mislovičová6Ivo Juránek7Ladislav Šoltés8Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, SK-84104 Bratislava, SlovakiaInstitute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, SK-84104 Bratislava, SlovakiaPolymer Institute, Slovak Academy of Sciences, SK-84541 Bratislava, SlovakiaPolymer Institute, Slovak Academy of Sciences, SK-84541 Bratislava, SlovakiaPolymer Institute, Slovak Academy of Sciences, SK-84541 Bratislava, SlovakiaIstituto per lo Studio delle Macromolecole, Consiglio Nazionale delle Ricerche, I-20133 Milano, ItalyInstitute of Chemistry, Slovak Academy of Science, SK-84538 Bratislava, SlovakiaInstitute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, SK-84104 Bratislava, SlovakiaInstitute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, SK-84104 Bratislava, SlovakiaThe bucillamine effect on free-radical-mediated degradation of high-molar-mass hyaluronan (HA) has been elucidated. As HA fragmentation is expected to decrease its dynamic viscosity, rotational viscometry was applied to follow the oxidative HA degradation. Non-isothermal chemiluminometry, thermogravimetry, differential scanning calorimetry, and size-exclusion chromatography (SEC) were applied to characterize resulting HA fragments. Although bucillamine completely inhibited the HA viscosity decrease caused by oxidative system, indicating HA protection from degradation, SEC analysis suggested that some other mechanisms leading to the bucillamine transformations without the decay of the viscosity may come into a play as well. Nonetheless, the link between the reduction of chemiluminescence intensity and disappearance of the differential scanning calorimetry exotherm at 270 °C for fragmented HAs indicates a particular role of the bucillamine in preventing the decrease of HA viscosity.http://www.mdpi.com/2073-4360/6/10/2625disease-modifying antirheumatic drugsrotational viscometrysize exclusion chromatographythermo-analytical methods |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mária Baňasová Katarína Valachová Jozef Rychlý Ivica Janigová Katarína Csomorová Raniero Mendichi Danica Mislovičová Ivo Juránek Ladislav Šoltés |
spellingShingle |
Mária Baňasová Katarína Valachová Jozef Rychlý Ivica Janigová Katarína Csomorová Raniero Mendichi Danica Mislovičová Ivo Juránek Ladislav Šoltés Effect of Bucillamine on Free-Radical-Mediated Degradation of High-Molar-Mass Hyaluronan Induced in vitro by Ascorbic Acid and Cu(II) Ions Polymers disease-modifying antirheumatic drugs rotational viscometry size exclusion chromatography thermo-analytical methods |
author_facet |
Mária Baňasová Katarína Valachová Jozef Rychlý Ivica Janigová Katarína Csomorová Raniero Mendichi Danica Mislovičová Ivo Juránek Ladislav Šoltés |
author_sort |
Mária Baňasová |
title |
Effect of Bucillamine on Free-Radical-Mediated Degradation of High-Molar-Mass Hyaluronan Induced in vitro by Ascorbic Acid and Cu(II) Ions |
title_short |
Effect of Bucillamine on Free-Radical-Mediated Degradation of High-Molar-Mass Hyaluronan Induced in vitro by Ascorbic Acid and Cu(II) Ions |
title_full |
Effect of Bucillamine on Free-Radical-Mediated Degradation of High-Molar-Mass Hyaluronan Induced in vitro by Ascorbic Acid and Cu(II) Ions |
title_fullStr |
Effect of Bucillamine on Free-Radical-Mediated Degradation of High-Molar-Mass Hyaluronan Induced in vitro by Ascorbic Acid and Cu(II) Ions |
title_full_unstemmed |
Effect of Bucillamine on Free-Radical-Mediated Degradation of High-Molar-Mass Hyaluronan Induced in vitro by Ascorbic Acid and Cu(II) Ions |
title_sort |
effect of bucillamine on free-radical-mediated degradation of high-molar-mass hyaluronan induced in vitro by ascorbic acid and cu(ii) ions |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2014-10-01 |
description |
The bucillamine effect on free-radical-mediated degradation of high-molar-mass hyaluronan (HA) has been elucidated. As HA fragmentation is expected to decrease its dynamic viscosity, rotational viscometry was applied to follow the oxidative HA degradation. Non-isothermal chemiluminometry, thermogravimetry, differential scanning calorimetry, and size-exclusion chromatography (SEC) were applied to characterize resulting HA fragments. Although bucillamine completely inhibited the HA viscosity decrease caused by oxidative system, indicating HA protection from degradation, SEC analysis suggested that some other mechanisms leading to the bucillamine transformations without the decay of the viscosity may come into a play as well. Nonetheless, the link between the reduction of chemiluminescence intensity and disappearance of the differential scanning calorimetry exotherm at 270 °C for fragmented HAs indicates a particular role of the bucillamine in preventing the decrease of HA viscosity. |
topic |
disease-modifying antirheumatic drugs rotational viscometry size exclusion chromatography thermo-analytical methods |
url |
http://www.mdpi.com/2073-4360/6/10/2625 |
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