Acute Synthesis of CPEB Is Required for Plasticity of Visual Avoidance Behavior in Xenopus
Neural plasticity requires protein synthesis, but the identity of newly synthesized proteins generated in response to plasticity-inducing stimuli remains unclear. We used in vivo bio-orthogonal noncanonical amino acid tagging (BONCAT) with the methionine analog azidohomoalanine (AHA) combined with t...
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doaj-3bdb66b2691840dbaed9ec8f20c6a5cc2020-11-24T21:54:51ZengElsevierCell Reports2211-12472014-02-016473774710.1016/j.celrep.2014.01.024Acute Synthesis of CPEB Is Required for Plasticity of Visual Avoidance Behavior in XenopusWanhua Shen0Han-Hsuan Liu1Lucio Schiapparelli2Daniel McClatchy3Hai-yan He4John R. Yates III5Hollis T. Cline6Key Lab of Organ Development and Regeneration of Zhejiang Province, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, ChinaThe Dorris Neuroscience Center, Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USAThe Dorris Neuroscience Center, Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USADepartment of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USAThe Dorris Neuroscience Center, Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USADepartment of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USAThe Dorris Neuroscience Center, Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USANeural plasticity requires protein synthesis, but the identity of newly synthesized proteins generated in response to plasticity-inducing stimuli remains unclear. We used in vivo bio-orthogonal noncanonical amino acid tagging (BONCAT) with the methionine analog azidohomoalanine (AHA) combined with the multidimensional protein identification technique (MudPIT) to identify proteins that are synthesized in the tadpole brain over 24 hr. We induced conditioning-dependent plasticity of visual avoidance behavior, which required N-methyl-D-aspartate (NMDA) and Ca2+-permeable α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, αCaMKII, and rapid protein synthesis. Combining BONCAT with western blots revealed that proteins including αCaMKII, MEK1, CPEB, and GAD65 are synthesized during conditioning. Acute synthesis of CPEB during conditioning is required for behavioral plasticity as well as conditioning-induced synaptic and structural plasticity in the tectal circuit. We outline a signaling pathway that regulates protein-synthesis-dependent behavioral plasticity in intact animals, identify newly synthesized proteins induced by visual experience, and demonstrate a requirement for acute synthesis of CPEB in plasticity.http://www.sciencedirect.com/science/article/pii/S2211124714000412 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wanhua Shen Han-Hsuan Liu Lucio Schiapparelli Daniel McClatchy Hai-yan He John R. Yates III Hollis T. Cline |
spellingShingle |
Wanhua Shen Han-Hsuan Liu Lucio Schiapparelli Daniel McClatchy Hai-yan He John R. Yates III Hollis T. Cline Acute Synthesis of CPEB Is Required for Plasticity of Visual Avoidance Behavior in Xenopus Cell Reports |
author_facet |
Wanhua Shen Han-Hsuan Liu Lucio Schiapparelli Daniel McClatchy Hai-yan He John R. Yates III Hollis T. Cline |
author_sort |
Wanhua Shen |
title |
Acute Synthesis of CPEB Is Required for Plasticity of Visual Avoidance Behavior in Xenopus |
title_short |
Acute Synthesis of CPEB Is Required for Plasticity of Visual Avoidance Behavior in Xenopus |
title_full |
Acute Synthesis of CPEB Is Required for Plasticity of Visual Avoidance Behavior in Xenopus |
title_fullStr |
Acute Synthesis of CPEB Is Required for Plasticity of Visual Avoidance Behavior in Xenopus |
title_full_unstemmed |
Acute Synthesis of CPEB Is Required for Plasticity of Visual Avoidance Behavior in Xenopus |
title_sort |
acute synthesis of cpeb is required for plasticity of visual avoidance behavior in xenopus |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2014-02-01 |
description |
Neural plasticity requires protein synthesis, but the identity of newly synthesized proteins generated in response to plasticity-inducing stimuli remains unclear. We used in vivo bio-orthogonal noncanonical amino acid tagging (BONCAT) with the methionine analog azidohomoalanine (AHA) combined with the multidimensional protein identification technique (MudPIT) to identify proteins that are synthesized in the tadpole brain over 24 hr. We induced conditioning-dependent plasticity of visual avoidance behavior, which required N-methyl-D-aspartate (NMDA) and Ca2+-permeable α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, αCaMKII, and rapid protein synthesis. Combining BONCAT with western blots revealed that proteins including αCaMKII, MEK1, CPEB, and GAD65 are synthesized during conditioning. Acute synthesis of CPEB during conditioning is required for behavioral plasticity as well as conditioning-induced synaptic and structural plasticity in the tectal circuit. We outline a signaling pathway that regulates protein-synthesis-dependent behavioral plasticity in intact animals, identify newly synthesized proteins induced by visual experience, and demonstrate a requirement for acute synthesis of CPEB in plasticity. |
url |
http://www.sciencedirect.com/science/article/pii/S2211124714000412 |
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