Anti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosis
Abstract Background Breast cancer is the most prevalent cancer among women. In triple-negative breast cancer (TNBC) cells, a novel quinone derivative, coenzyme Q0 (CoQ0), promotes apoptosis and cell-cycle arrest. This study explored the anti-epithelial–mesenchymal transition (EMT) and antimetastatic...
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doaj-3bdbe25747804e4682c0c299dac373c02020-11-25T03:27:01ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662019-05-0138112110.1186/s13046-019-1196-xAnti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosisHsin-Ling Yang0Varadharajan Thiyagarajan1Pei-Chun Shen2Dony Chacko Mathew3Kai-Yuan Lin4Jiunn-Wang Liao5You-Cheng Hseu6Institute of Nutrition, College of Biopharmaceuticals and Food Sciences, China Medical UniversityDepartment of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical UniversityInstitute of Nutrition, College of Biopharmaceuticals and Food Sciences, China Medical UniversityInstitute of Nutrition, College of Biopharmaceuticals and Food Sciences, China Medical UniversityDepartment of Medical Research, Chi-Mei Medical CenterGraduate Institute of Veterinary Pathology, National Chung Hsing UniversityDepartment of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical UniversityAbstract Background Breast cancer is the most prevalent cancer among women. In triple-negative breast cancer (TNBC) cells, a novel quinone derivative, coenzyme Q0 (CoQ0), promotes apoptosis and cell-cycle arrest. This study explored the anti-epithelial–mesenchymal transition (EMT) and antimetastatic attributes of CoQ0 in TNBC (MDA-MB-231). Methods Invasion, as well as MTT assays were conducted. Lipofectamine RNAiMAX was used to transfect cells with β-catenin siRNA. Through Western blotting and RT-PCR, the major signaling pathways’ protein expressions were examined, and the biopsied tumor tissues underwent immunohistochemical and hematoxylin and eosin staining as well as Western blotting. Results CoQ0 (0.5–2 μM) hindered tumor migration, invasion, and progression. Additionally, it caused MMP-2/− 9, uPA, uPAR, and VEGF downregulation. Furthermore, in highly metastatic MDA-MB-231 cells, TIMP-1/2 expression was subsequently upregulated and MMP-9 expression was downregulated. In addition, CoQ0 inhibited metastasis and EMT in TGF-β/TNF-α-stimulated non-tumorigenic MCF-10A cells. Bioluminescence imaging of MDA-MB-231 luciferase–injected live mice demonstrated that CoQ0 significantly inhibited metastasis of the breast cancer to the lungs and inhibited the development of tumors in MDA-MB-231 xenografted nude mice. Silencing of β-catenin with siRNA stimulated CoQ0-inhibited EMT. Western blotting as well as histological analysis established that CoQ0 reduced xenografted tumor development because apoptosis induction, cell-cycle inhibition, E-cadherin upregulation, β-catenin downregulation, and metastasis and EMT regulatory protein modulation were observed. Conclusions CoQ0 inhibited the progression of metastasis as well as EMT (in vitro and in vivo). The described approach has potential in treating human breast cancer metastasis.http://link.springer.com/article/10.1186/s13046-019-1196-xCoQ0TNBCEMTMetastasisHuman epidermal growth factor receptor 2TGF-β1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hsin-Ling Yang Varadharajan Thiyagarajan Pei-Chun Shen Dony Chacko Mathew Kai-Yuan Lin Jiunn-Wang Liao You-Cheng Hseu |
spellingShingle |
Hsin-Ling Yang Varadharajan Thiyagarajan Pei-Chun Shen Dony Chacko Mathew Kai-Yuan Lin Jiunn-Wang Liao You-Cheng Hseu Anti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosis Journal of Experimental & Clinical Cancer Research CoQ0 TNBC EMT Metastasis Human epidermal growth factor receptor 2 TGF-β1 |
author_facet |
Hsin-Ling Yang Varadharajan Thiyagarajan Pei-Chun Shen Dony Chacko Mathew Kai-Yuan Lin Jiunn-Wang Liao You-Cheng Hseu |
author_sort |
Hsin-Ling Yang |
title |
Anti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosis |
title_short |
Anti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosis |
title_full |
Anti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosis |
title_fullStr |
Anti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosis |
title_full_unstemmed |
Anti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosis |
title_sort |
anti-emt properties of coq0 attributed to pi3k/akt/nfkb/mmp-9 signaling pathway through ros-mediated apoptosis |
publisher |
BMC |
series |
Journal of Experimental & Clinical Cancer Research |
issn |
1756-9966 |
publishDate |
2019-05-01 |
description |
Abstract Background Breast cancer is the most prevalent cancer among women. In triple-negative breast cancer (TNBC) cells, a novel quinone derivative, coenzyme Q0 (CoQ0), promotes apoptosis and cell-cycle arrest. This study explored the anti-epithelial–mesenchymal transition (EMT) and antimetastatic attributes of CoQ0 in TNBC (MDA-MB-231). Methods Invasion, as well as MTT assays were conducted. Lipofectamine RNAiMAX was used to transfect cells with β-catenin siRNA. Through Western blotting and RT-PCR, the major signaling pathways’ protein expressions were examined, and the biopsied tumor tissues underwent immunohistochemical and hematoxylin and eosin staining as well as Western blotting. Results CoQ0 (0.5–2 μM) hindered tumor migration, invasion, and progression. Additionally, it caused MMP-2/− 9, uPA, uPAR, and VEGF downregulation. Furthermore, in highly metastatic MDA-MB-231 cells, TIMP-1/2 expression was subsequently upregulated and MMP-9 expression was downregulated. In addition, CoQ0 inhibited metastasis and EMT in TGF-β/TNF-α-stimulated non-tumorigenic MCF-10A cells. Bioluminescence imaging of MDA-MB-231 luciferase–injected live mice demonstrated that CoQ0 significantly inhibited metastasis of the breast cancer to the lungs and inhibited the development of tumors in MDA-MB-231 xenografted nude mice. Silencing of β-catenin with siRNA stimulated CoQ0-inhibited EMT. Western blotting as well as histological analysis established that CoQ0 reduced xenografted tumor development because apoptosis induction, cell-cycle inhibition, E-cadherin upregulation, β-catenin downregulation, and metastasis and EMT regulatory protein modulation were observed. Conclusions CoQ0 inhibited the progression of metastasis as well as EMT (in vitro and in vivo). The described approach has potential in treating human breast cancer metastasis. |
topic |
CoQ0 TNBC EMT Metastasis Human epidermal growth factor receptor 2 TGF-β1 |
url |
http://link.springer.com/article/10.1186/s13046-019-1196-x |
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