PEI-Mediated Transient Transfection of High Five Cells at Bioreactor Scale for HIV-1 VLP Production

PEI-Mediated Transient Transfection of High Five Cells at Bioreactor Scale for HIV-1 VLP Production

High Five cells are an excellent host for the production of virus-like particles (VLPs) with the baculovirus expression vector system (BEVS). However, the concurrent production of high titers of baculovirus hinder the purification of these nanoparticles due to similarities in their physicochemical p...

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Bibliographic Details
Main Authors: Eduard Puente-Massaguer, Florian Strobl, Reingard Grabherr, Gerald Striedner, Martí Lecina, Francesc Gòdia
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Nanomaterials
Subjects:
High Five cells
transient gene expression
polyethylenimine
virus-like particle
bioreactor
Online Access:https://www.mdpi.com/2079-4991/10/8/1580

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https://www.mdpi.com/2079-4991/10/8/1580

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