Global Reorganization of the Nuclear Landscape in Senescent Cells
Cellular senescence has been implicated in tumor suppression, development, and aging and is accompanied by large-scale chromatin rearrangements, forming senescence-associated heterochromatic foci (SAHF). However, how the chromatin is reorganized during SAHF formation is poorly understood. Furthermor...
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doaj-3c24731ad6c0489a928431702a77252b2020-11-24T23:54:39ZengElsevierCell Reports2211-12472015-02-0110447148310.1016/j.celrep.2014.12.055Global Reorganization of the Nuclear Landscape in Senescent CellsTamir Chandra0Philip Andrew Ewels1Stefan Schoenfelder2Mayra Furlan-Magaril3Steven William Wingett4Kristina Kirschner5Jean-Yves Thuret6Simon Andrews7Peter Fraser8Wolf Reik9Epigenetics Programme, The Babraham Institute, Cambridge CB22 3AT, UKEpigenetics Programme, The Babraham Institute, Cambridge CB22 3AT, UKNuclear Dynamics Programme, The Babraham Institute, Cambridge CB22 3AT, UKNuclear Dynamics Programme, The Babraham Institute, Cambridge CB22 3AT, UKNuclear Dynamics Programme, The Babraham Institute, Cambridge CB22 3AT, UKCambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UKCEA, iBiTec-S, SBIGeM/CNRS FRE3377 I2BC/Université Paris-Sud, Gif-sur-Yvette 91191, FranceBioinformatics Group, The Babraham Institute, Cambridge CB22 3AT, UKNuclear Dynamics Programme, The Babraham Institute, Cambridge CB22 3AT, UKEpigenetics Programme, The Babraham Institute, Cambridge CB22 3AT, UKCellular senescence has been implicated in tumor suppression, development, and aging and is accompanied by large-scale chromatin rearrangements, forming senescence-associated heterochromatic foci (SAHF). However, how the chromatin is reorganized during SAHF formation is poorly understood. Furthermore, heterochromatin formation in senescence appears to contrast with loss of heterochromatin in Hutchinson-Gilford progeria. We mapped architectural changes in genome organization in cellular senescence using Hi-C. Unexpectedly, we find a dramatic sequence- and lamin-dependent loss of local interactions in heterochromatin. This change in local connectivity resolves the paradox of opposing chromatin changes in senescence and progeria. In addition, we observe a senescence-specific spatial clustering of heterochromatic regions, suggesting a unique second step required for SAHF formation. Comparison of embryonic stem cells (ESCs), somatic cells, and senescent cells shows a unidirectional loss in local chromatin connectivity, suggesting that senescence is an endpoint of the continuous nuclear remodelling process during differentiation.http://www.sciencedirect.com/science/article/pii/S221112471401122X |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tamir Chandra Philip Andrew Ewels Stefan Schoenfelder Mayra Furlan-Magaril Steven William Wingett Kristina Kirschner Jean-Yves Thuret Simon Andrews Peter Fraser Wolf Reik |
spellingShingle |
Tamir Chandra Philip Andrew Ewels Stefan Schoenfelder Mayra Furlan-Magaril Steven William Wingett Kristina Kirschner Jean-Yves Thuret Simon Andrews Peter Fraser Wolf Reik Global Reorganization of the Nuclear Landscape in Senescent Cells Cell Reports |
author_facet |
Tamir Chandra Philip Andrew Ewels Stefan Schoenfelder Mayra Furlan-Magaril Steven William Wingett Kristina Kirschner Jean-Yves Thuret Simon Andrews Peter Fraser Wolf Reik |
author_sort |
Tamir Chandra |
title |
Global Reorganization of the Nuclear Landscape in Senescent Cells |
title_short |
Global Reorganization of the Nuclear Landscape in Senescent Cells |
title_full |
Global Reorganization of the Nuclear Landscape in Senescent Cells |
title_fullStr |
Global Reorganization of the Nuclear Landscape in Senescent Cells |
title_full_unstemmed |
Global Reorganization of the Nuclear Landscape in Senescent Cells |
title_sort |
global reorganization of the nuclear landscape in senescent cells |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2015-02-01 |
description |
Cellular senescence has been implicated in tumor suppression, development, and aging and is accompanied by large-scale chromatin rearrangements, forming senescence-associated heterochromatic foci (SAHF). However, how the chromatin is reorganized during SAHF formation is poorly understood. Furthermore, heterochromatin formation in senescence appears to contrast with loss of heterochromatin in Hutchinson-Gilford progeria. We mapped architectural changes in genome organization in cellular senescence using Hi-C. Unexpectedly, we find a dramatic sequence- and lamin-dependent loss of local interactions in heterochromatin. This change in local connectivity resolves the paradox of opposing chromatin changes in senescence and progeria. In addition, we observe a senescence-specific spatial clustering of heterochromatic regions, suggesting a unique second step required for SAHF formation. Comparison of embryonic stem cells (ESCs), somatic cells, and senescent cells shows a unidirectional loss in local chromatin connectivity, suggesting that senescence is an endpoint of the continuous nuclear remodelling process during differentiation. |
url |
http://www.sciencedirect.com/science/article/pii/S221112471401122X |
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