Cathelicidin Insufficiency in Patients with Fatal Leptospirosis.
Leptospirosis causes significant morbidity and mortality worldwide; however, the role of the host immune response in disease progression and high case fatality (>10-50%) is poorly understood. We conducted a multi-parameter investigation of patients with acute leptospirosis to identify mechanisms...
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2016-11-01
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doaj-3c2aea0885d74fd99f3d6b03ab5fe8b22021-04-21T17:45:03ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742016-11-011211e100594310.1371/journal.ppat.1005943Cathelicidin Insufficiency in Patients with Fatal Leptospirosis.Janet C LindowElsio A WunderStephen J PopperJin-Na MinPraveen MannamAnup SrivastavaYi YaoKathryn P HackerKhadir RaddassiPatty J LeeRuth R MontgomeryAlbert C ShawJose E HaganGuilherme C AraújoNivison NeryDavid A RelmanCharles C KimMitermayer G ReisAlbert I KoLeptospirosis causes significant morbidity and mortality worldwide; however, the role of the host immune response in disease progression and high case fatality (>10-50%) is poorly understood. We conducted a multi-parameter investigation of patients with acute leptospirosis to identify mechanisms associated with case fatality. Whole blood transcriptional profiling of 16 hospitalized Brazilian patients with acute leptospirosis (13 survivors, 3 deceased) revealed fatal cases had lower expression of the antimicrobial peptide, cathelicidin, and chemokines, but more abundant pro-inflammatory cytokine receptors. In contrast, survivors generated strong adaptive immune signatures, including transcripts relevant to antigen presentation and immunoglobulin production. In an independent cohort (23 survivors, 22 deceased), fatal cases had higher bacterial loads (P = 0.0004) and lower anti-Leptospira antibody titers (P = 0.02) at the time of hospitalization, independent of the duration of illness. Low serum cathelicidin and RANTES levels during acute illness were independent risk factors for higher bacterial loads (P = 0.005) and death (P = 0.04), respectively. To investigate the mechanism of cathelicidin in patients surviving acute disease, we administered LL-37, the active peptide of cathelicidin, in a hamster model of lethal leptospirosis and found it significantly decreased bacterial loads and increased survival. Our findings indicate that the host immune response plays a central role in severe leptospirosis disease progression. While drawn from a limited study size, significant conclusions include that poor clinical outcomes are associated with high systemic bacterial loads, and a decreased antibody response. Furthermore, our data identified a key role for the antimicrobial peptide, cathelicidin, in mounting an effective bactericidal response against the pathogen, which represents a valuable new therapeutic approach for leptospirosis.https://doi.org/10.1371/journal.ppat.1005943 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Janet C Lindow Elsio A Wunder Stephen J Popper Jin-Na Min Praveen Mannam Anup Srivastava Yi Yao Kathryn P Hacker Khadir Raddassi Patty J Lee Ruth R Montgomery Albert C Shaw Jose E Hagan Guilherme C Araújo Nivison Nery David A Relman Charles C Kim Mitermayer G Reis Albert I Ko |
spellingShingle |
Janet C Lindow Elsio A Wunder Stephen J Popper Jin-Na Min Praveen Mannam Anup Srivastava Yi Yao Kathryn P Hacker Khadir Raddassi Patty J Lee Ruth R Montgomery Albert C Shaw Jose E Hagan Guilherme C Araújo Nivison Nery David A Relman Charles C Kim Mitermayer G Reis Albert I Ko Cathelicidin Insufficiency in Patients with Fatal Leptospirosis. PLoS Pathogens |
author_facet |
Janet C Lindow Elsio A Wunder Stephen J Popper Jin-Na Min Praveen Mannam Anup Srivastava Yi Yao Kathryn P Hacker Khadir Raddassi Patty J Lee Ruth R Montgomery Albert C Shaw Jose E Hagan Guilherme C Araújo Nivison Nery David A Relman Charles C Kim Mitermayer G Reis Albert I Ko |
author_sort |
Janet C Lindow |
title |
Cathelicidin Insufficiency in Patients with Fatal Leptospirosis. |
title_short |
Cathelicidin Insufficiency in Patients with Fatal Leptospirosis. |
title_full |
Cathelicidin Insufficiency in Patients with Fatal Leptospirosis. |
title_fullStr |
Cathelicidin Insufficiency in Patients with Fatal Leptospirosis. |
title_full_unstemmed |
Cathelicidin Insufficiency in Patients with Fatal Leptospirosis. |
title_sort |
cathelicidin insufficiency in patients with fatal leptospirosis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2016-11-01 |
description |
Leptospirosis causes significant morbidity and mortality worldwide; however, the role of the host immune response in disease progression and high case fatality (>10-50%) is poorly understood. We conducted a multi-parameter investigation of patients with acute leptospirosis to identify mechanisms associated with case fatality. Whole blood transcriptional profiling of 16 hospitalized Brazilian patients with acute leptospirosis (13 survivors, 3 deceased) revealed fatal cases had lower expression of the antimicrobial peptide, cathelicidin, and chemokines, but more abundant pro-inflammatory cytokine receptors. In contrast, survivors generated strong adaptive immune signatures, including transcripts relevant to antigen presentation and immunoglobulin production. In an independent cohort (23 survivors, 22 deceased), fatal cases had higher bacterial loads (P = 0.0004) and lower anti-Leptospira antibody titers (P = 0.02) at the time of hospitalization, independent of the duration of illness. Low serum cathelicidin and RANTES levels during acute illness were independent risk factors for higher bacterial loads (P = 0.005) and death (P = 0.04), respectively. To investigate the mechanism of cathelicidin in patients surviving acute disease, we administered LL-37, the active peptide of cathelicidin, in a hamster model of lethal leptospirosis and found it significantly decreased bacterial loads and increased survival. Our findings indicate that the host immune response plays a central role in severe leptospirosis disease progression. While drawn from a limited study size, significant conclusions include that poor clinical outcomes are associated with high systemic bacterial loads, and a decreased antibody response. Furthermore, our data identified a key role for the antimicrobial peptide, cathelicidin, in mounting an effective bactericidal response against the pathogen, which represents a valuable new therapeutic approach for leptospirosis. |
url |
https://doi.org/10.1371/journal.ppat.1005943 |
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