Expression, purification and low-resolution structure of human vitamin C transporter SVCT1 (SLC23A1).

Expression and purification of human membrane proteins for structural studies represent a great challenge. This is because micro- to milligram amounts of pure isolated protein are required. To this aim, we successfully expressed the human vitamin C transporter-1 (hSVCT1; SLC23A1) in Xenopus laevis o...

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Bibliographic Details
Main Authors: Rajendra Boggavarapu, Jean-Marc Jeckelmann, Daniel Harder, Philipp Schneider, Zöhre Ucurum, Matthias Hediger, Dimitrios Fotiadis
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3790709?pdf=render